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  1. 12. If you cannot obtain any more blood from the rabbit and the heart is still beating, remove the needle and insert another, in the case of a blockage. 13. The minimum acceptable amount of blood allowed for a researcher is 80mls, however, more is preferable (above 100mls) depending on the size of the rabbit. 14.

  2. 5 de mar. de 2023 · Inspect your needle for defects. The end should have no obstructions or hooks that will restrict the flow of blood. 2. Thread the needle into the holder. Use the needle sheath to secure the needle in the holder. 3. Tap any tubes that contain additives to dislodge the additives from the walls of the tube. 4.

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  4. 1 de jul. de 2023 · The maximum permitted blood volume includes blood lost during collection. As a general rule, 20 drops = 1 mL (i.e. 5 drops = 250 uL) Maximal blood collection limits are as follows: No more than 1% of the animal’s body weight in one collection or over a 24 hour period. For example: 25g mouse x 1% = 0.25mL or 250uL maximum blood removal.

  5. Guidelines. An animal’s total blood volume can vary but is generally estimated as approximately 6-8% of body weight (Table 2). i. The circulating blood volume can generally be estimated as 55-70 ml/kg of total body weight. However, care should be taken in these calculations as the % of total blood volume will be lower (~15%) in sick, obese ...

  6. 18 de ago. de 2019 · The Rodent Nurse. · August 18, 2019 ·. Taking blood samples in our rabbit patients doesn’t have to be stressful or tricky! There are lots of things we can do to #reducestress for our patient, and, ourselves! Firstly, choosing the most suitable site for sampling is vital. Personally, I will always head straight for that lateral #saphenous vein!

  7. Step 6: Draw Blood. Perform venipuncture using the following steps: Place your thumb BELOW the venipuncture site to retract the skin. Be sure not to touch the venipuncture site or you will need to repeat the cleaning process. Puncture the vein quickly and at a 15 to 30 degree angle, with the bevel of the needle pointing up.

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