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  1. www.science.org › doi › 10Massively parallel CRISPR-assisted homologous recombination...

    15 de may. de 2024 · In this study, we introduce CRISPR-Cas9– and HDR-assisted saturation editing (CHASE) as a novel SGE method. We show that a homologous recombination donor–coupled CRISPR system using an SpG variant exhibited high codon replacement efficiencies for scalable gene editing.

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  3. www.mdpi.com › 2076/2607/12-6 › 1102The Influence of Homologous Arm Length on Homologous...

    Hace 4 días · Microorganisms | Free Full-Text | The Influence of Homologous Arm Length on Homologous Recombination Gene Editing Efficiency Mediated by SSB/CRISPR-Cas9 in Escherichia coli. Journals. Microorganisms. Volume 12. Issue 6. 10.3390/microorganisms12061102.

  4. www.nature.com › articles › s41467/024/48258-5Regulation of Myc transcription by an enhancer cluster ... -...

    10 de may. de 2024 · MYC plays various roles in pluripotent stem cells, including the promotion of somatic cell reprogramming to pluripotency, the regulation of cell competition and the control of embryonic diapause.

  5. forbes.es › forbes-ricos › 471453Estas son las 10 personas más ricas del mundo a 1 de junio de...

    Hace 1 día · Warren Buffett 8. Sergey Brin 9. Bill Gates 10. Steve Ballmer *A partir del 1 de junio de 2024 a las 12 a.m. ET. 1 ... En 2000, fusionó un banco en línea que cofundó, X.com, con una empresa similar cofundada por Peter Thiel para formar PayPal, que eBay compró en 2002 por 1.400 millones de dólares. Fundó SpaceX en 2002 en El ...

  6. www.sciencedirect.com › science › articleA rare inter-haplotypic recombination at the S -locus contributed...

    7 de may. de 2024 · In this study, sequence insertions 146 were also detected in the right and left flanking regions of the S-locus associated with 147 a recombination breakpoint (Supplementary Fig. 14; Supplementary Table 8) and 148 therefore, might be one of the important driving forces for this rare crossover event. 149 This haplotypic recombination occurred only in the Poncirus, possibly resulting from 150 a ...

  7. academic.oup.com › nar › advance-articleTranscription factor shapes chromosomal conformation and...

    8 de may. de 2024 · For recombination, 500-bp fragments of upstream and downstream target genes were used as homologous arms. Plasmid pMini was used as the base for mutation, which was derived from paternal pEZ15Asp. Gene complement was performed in Z. mobilis strains using plasmid pEZ15Asp with endogenous eno promoter.

  8. www.nature.com › articles › s41375/024/02259-xCre recombinase promotes leukemogenesis in the presence of both...

    8 de may. de 2024 · Cre recombinase murine models allow the expression of mutated genes in a cell type-specific manner or via an inducible mechanism and has revolutionized biomedical research. However, these models...