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  1. 28 de nov. de 2023 · BLAST compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. It can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

    • Blast

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Nucleotide BLAST

      Select the sequence database to run searches against. No...

    • Home

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Recent Results

      Click on an RID to view the corresponding BLAST report....

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

    • Help

      Welcome to BLAST Help¶ Getting started¶ Quick start guide....

    • Protein BLAST

      Protein BLAST - BLAST: Basic Local Alignment Search Tool

    • Blastx

      Blastx - BLAST: Basic Local Alignment Search Tool

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. Primer-BLAST is a tool that uses Primer3 and BLAST to design primers for PCR. You can enter a sequence, a range, or a refseq record as PCR template and adjust various parameters to find optimal primers.

  4. This document first introduces the BLAST databases available from NCBI (in Section 2). The actual guide (Section 3) divides BLAST searches into several categories according to the nature and size of the input query and the primary goal of the search.

  5. Learn about the new features and enhancements of BLAST, the popular tool for sequence similarity search. Find links to BLAST forms, databases, documentation, and recent results.

  6. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.

  7. 21 de mar. de 2024 · The Basic Local Alignment Search Tool (BLAST) finds regions of similarity between sequences. The program compares nucleotide or protein sequences and calculates the statistical significance of matches.

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