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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • En caché

   Maximum number of database sequences (with unique sequence identifier) Blast finds for primer-blast to screen for primer pair specificities. Note that the actual number of similarity regions (or the number of hits) may be much larger than this (for example, there may be a large number of hits on a single target sequence such as a chromosome).

2. www.ncbi.nlm.nih.gov › guide › howtoRun BLAST software on a local computer - National Center for ...

   www.ncbi.nlm.nih.gov › guide › howto

   • En caché

   How to: Run BLAST software on a local computer. In order to run BLAST locally on your own computer, you will need to download the BLAST+ software and databases. To see the instructions and get the latest version of BLAST+, go to the BLAST download page . NCBI Home.

3. blast.ncbi.nlm.nih.gov › doc › blast-helpWelcome to BLAST Help — BLASTHelp documentation

   blast.ncbi.nlm.nih.gov › doc › blast-help

   • En caché

   Welcome to BLAST Help¶ Getting started¶ Quick start guide. Quick start results guide. Guide to BLAST home and search pages. Blast report description. Blast topics. Frequently Asked Questions. Getting Help¶ Write to the help desk. Mailing list. BLAST tutorials. About BLAST¶ NCBI Handbook. The Statistics of Sequence Similarity Scores. BLAST ...

4. www.ncbi.nlm.nih.gov › refseqRefSeq: NCBI Reference Sequence Database - National Center for...

   www.ncbi.nlm.nih.gov › refseq

   • En caché

   RefSeq: NCBI Reference Sequence Database. A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

5. www.ncbi.nlm.nih.gov › blast › BlastBLAST: Basic Local Alignment Search Tool - National Center for...

   www.ncbi.nlm.nih.gov › blast › Blast

   • En caché

   28 de nov. de 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

6. blast.ncbi.nlm.nih.gov › BlastNucleotide BLAST: Search nucleotide databases using a nucleotide...

   blast.ncbi.nlm.nih.gov › Blast

   • En caché

   PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query. DELTA-BLAST constructs a PSSM using the results of a Conserved Domain Database search and searches a sequence database.

7. blast.ncbi.nlm.nih.gov › BlastProtein BLAST: search protein databases using a protein query

   blast.ncbi.nlm.nih.gov › Blast

   • En caché

   PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run. PHI-BLAST performs the search but limits alignments to those that match a pattern in the query. DELTA-BLAST constructs a PSSM using the results of a Conserved Domain Database search and searches a sequence database.